17β雌二醇对人牙周韧带细胞表达碱性磷酸酶和骨保护素因子的影响

Effects of 17β-estradiol on alkaline phosphatase and osteoprotegerin expression in human periodontal ligament cells

目的探讨17β雌二醇（17β-estradiol，17β-E2）对人牙周韧带细胞（human periodontal ligament cells，hPDLC）表达碱性磷酸酶（alkaline phosphatase，ALP）和骨保护素（osteoprotegerin，OPG）的影响。方法采用组织块法培养hPDLC，培养基中加入不同浓度17β-E2，对硝基酚磷酸酯法检测ALP活性；ELISA法和反转录聚合酶链反应（RT-PCR）法确定OPG含量。结果各浓度组17β-E2作用于hPDLC14d和21d后均可促进ALP的表达（P〈0．05）；生理浓度（1nmol/L）促进OPG蛋白分泌和mRNA表达（P〈0．05），72hOPG蛋白分泌量达（4714．89±71．48）ng／L；高浓度（100nmol/L）对OPG分泌无影响（P〉0．05），72hOPG蛋白分泌量为（1932．04±51．59）ng／L，但降低了OPGmRNA表达（P〈0．05）。结论17β-E2可能通过促进ALP和OPG的表达介导局部牙周组织的骨保护作用。

Objective To investigate the effects of 17β-estradiol on the expression of alkaline phosphatase（ALP） and osteoprotegerin in human periodontal ligament cells. Methods Human periodontal ligament cells （hPDLC） were obtained from periodontal tissue explants of teeth extracted for orthodontic treatment. ALP activity was determined by PNPP, and OPG protein and corresponding mRNA levels were quantitatively detected by ELISA and RT-PCR. Results ALP activity was significantly increased at 14 clays and 21 clays （ P 〈 0. 05 ）. 17β-E2 of physiological concentration promoted secretion of OPG protein and expression of OPG mRNA （ P 〈 0. 05 ）. 17β-E2 with high-dose showed no effect on OPG protein secretion and decrease OPG mRNA expression. Conclusions 17β-E2 may have a positive impact on periodontium through promoting expression of ALP and OPG in hPDLC.