摘要
目的:建立脑脉通有效部位中的川芎嗪的含量测定方法。方法:采用大连依利特Hypersil ODS2 C18色谱柱(250mm×4.6mm,5μm),流动相为甲醇—水溶液(30∶70),流速:1.0ml/min,检测波长:316nm。结果:川芎嗪平均回收率为99.85%,RSD为1.48%。结论:所建立的方法简便、准确,可作为有效部位的质量控制方法。
Objective: to establish RP- HPLC method for determination of Ligustrazine in effective parts of Naomaitong. Methods: Using Hypersil ODS2 C18 column (250 mm×4.6 mm, 5μm) of Dalian Elite, the mobile phase was a mixture of methanol-water (30:70). The flow rate was 1.0 ml/min, the wavelength was 316 nm. Results: The average recovery rate was 99.85 %, RSD= 1.48% ; Conclusion: The method is simple,accurate and can be used to control the quality of effective parrs.
出处
《河南中医学院学报》
2008年第1期39-40,共2页
Journal of Henan University of Chinese Medicine
基金
国家自然科学基金资助项目(编号:30371812)