紫外线诱变提高细菌产纤维素酶活力的研究

Study on Mutation of Cellulase-Decomposing Bacteria Induced by UV to Increase Cellulase Activity

从南京红山动物园土样及食草动物的粪便中分离出14种产纤维素酶菌株，其中F1表现出较高的酶活力。以F1为出发菌株，通过紫外线诱变处理，采用透明圈法初筛和摇瓶培养复筛，获得了10株高产纤维素酶的突变株Q1～Q10。经紫外线诱变处理的Q3突变株产酶活力最高，与出发菌株相比酶活力提高了15．8倍。

Fourteen strains of cellulase-decomposing microorganisms were isolated from excreta samples of herbivores and soil in Nanjing Hongshan Zoo. Among them,strain F1 had the highest enzyme activity. Strain F1 was used as the original strain and treated with UV. Ten cellulase-producing mutants Q1-Q10 were obtained by transparent zone method and shaking flask cuhure method. The results showed that the cellulase-producing ability of Q3 increased 15.8 times compared to that of the original strain.