HPLC法同时测定茵山莲颗粒中绿原酸和原儿茶酸的含量

Determination of chlorogenic acid and protocatechuic acid in Yinshanlian granules by HPLC

目的建立同时测定茵山莲颗粒中绿原酸和原儿茶酸含量的方法。方法采用HPLC法。色谱柱为DiamonsiL C18柱（4．6mm×200mm，5μm），流动相为乙腈-质量分数为0．2％的磷酸水溶液（体积比10：90），流速为1．0mL·min^-1，柱温为30℃，检测波长为285nm，进样量为20μL。结果绿原酸与原儿茶酸质量浓度分别在4．80～43．2mg·L^-1、4．08～36．7mg·L^-1内与峰面积呈良好的线性关系，平均回收率分别为101．8％（RSD=1．0％，n=9）和98．5％（RSD=1．4％，n=9）。结论该方法操作简便准确，重复性好，可作为茵山莲颗粒质量控制方法之一。

Objective To develop an HPLC method for the simultaneous determination of chlorogenic acid and protocatechuic acid in Yinshanlian （traditional Chinese medicines） granules. Methods Chromatography was performed on a Diamonsil C18 column （200 mm × 4.6 ram, 5μm） with the mobile phase of acetonitrile- 0.2 % phosphoric acid solution（ W： W = 10：90）. The detection wavelength was set at 285 nm and the column temperature was 30 ℃. Results The linear calibration curve were obtained in the concentration range of 4.80 - 43.2 mg· L^- 1 for chlorogenic acid and 4.08 - 36.7 mg· L^- 1 for protocatechuic acid. The mean recoveries of chlorogenic acid and protocatechuic acid were 101.8 % （RSD = 1.0 %, n = 9） and 98.5 % （RSD = 1.4 %, n = 9）, respectively. Conclusions The method can be used to control the quality of Yinshanlian granules, which provides the advantage of quickness, simplicity and repeatability.