松花粉对前列腺增生大鼠体内微量元素锌、铜代谢的影响

Effect of Masson Pine Pollen on Metabolism of Trace Elements Zinc and Copper in Rats with Benign Prostatic Hypertrophy

目的观察马尾松花粉(松花粉)对前列腺增生大鼠体内微量元素代谢的影响。方法24只雄性Spra-gue-Dawley大鼠分为对照组、安慰剂组和松花粉组,每组8只。始终给对照组大鼠饲喂普通饲料;每日投料前分别给安慰剂组和松花粉组大鼠饲喂安慰剂(淀粉片)和松花粉片,3组大鼠的进食量完全相同。适应2周后开始给对照组大鼠肌肉注射橄榄油1ml.kg-1.d-1,给其余两组大鼠肌肉注射橄榄油配制的丙酸睾酮注射液4mg.kg-1.d-1,持续2周后停止注射。继续饲喂4周后将大鼠全部处死。取全血、肝脏和前列腺组织,称重前列腺组织,计算前列腺指数,用苏木精-伊红染色检测前列腺腺体增生情况,测定微量元素铜、锌的含量。结果松花粉组大鼠的前列腺重量[(1062.3±91.9)mgvs.(1127.3±111.2)mg]及前列腺指数[(3.18±0.31)mg/gvs.(3.26±0.31)mg/g]均低于安慰剂组。安慰剂组前列腺腺体的乳头状增生比率(60.0%±10.0%)显著高于松花粉组(20.0%±10.0%)(P<0.05)。两组的血清铜、锌含量均显著低于对照组(P<0.05)。松花粉组大鼠的肝脏锌含量显著低于安慰剂组(P<0.05),与对照组比较无显著差异;前列腺锌、铜含量均显著高于其他两组(P<0.05)。结论松花粉对大鼠前列腺增生有明显抑制作用,调节体内微量元素锌、铜的代谢可能是其作用机理之一。

Objective To study the effect of masson pine pollen on the metabolism of trace elements zinc and copper in rats with benign prostatic hypertrophy （BPH）. Methods Totally 24 male Sprague-Dawley rats were randomly and equally divided into control group, placebo group, and masson pine pollen group. Rats in the control group were fed with normal diets, while rats in the placebo group and the masson pine pollen group were fed with starch tablets and masson pine pollen, respectively, before fed with normal diets. After 2 weeks, rats in the control group was injected with olive oil （ 1 ml · kg^-1 · d^ -1 ）. Meanwhile, rats in the placebo group and the masson pine pollen group were injected with testosterone propionate （4 mg· kg ^- 1 ·d ^- 1 ） , which was dissolved in olive oil. The injection period lasted 2 weeks to establish the BPH models. After having being fed for another 4 weeks, all rats were killed and the contents of zinc and copper in their sera, livers, and prostates were measured. Results The prostate weight [ （1062.3±91.9） mgvs. （1127.3±111.2） mg] andpros- tate index [ （3. 18 ±0. 31 ） mg/g vs. （3.26 ±0. 31 ） mg/g] in the masson pine pollen group were significantly lower than in the placebo group. The papillary hyperplasia ratio in the placebo group （ 60.0% ± 10.0% ） was significantly higher than in the masson pine pollen group （20.0% ± 10.0% ） （P 〈0.05）. The serum zinc and copper contents in two model groups were significantly lower than in the control group （ P 〈 0.05 ）. The liver zinc level was significantly lower in the masson pine pollen group than in placebo group （ P 〈 0.05 ）, but was no significantly different when compared with the control group. However, the prostate zinc and copper contents were highest in the masson pine pollen group among these three groups （ P 〈 0.05 ）. Conclusions Masson pine pollen can remarkably inhibit BPH in rats. One possible mechanism may involve its regulatory effect on the metabolism of zinc and copper.