子宫内膜异位症组织中MIF及VEGF蛋白的表达及意义

Expression of MIF and VEGF protein and the significance in endometriosis

目的:研究巨噬细胞移动抑制因子及血管内皮生长因子在子宫内膜异位症中的表达及相关性,并探讨其临床意义。方法:应用免疫组化SP法检测巨噬细胞移动抑制因子及血管内皮生长因子在30例子宫内膜异位症患者异位内膜、在位内膜及30例正常对照组子宫内膜的表达。结果:巨噬细胞移动抑制因子主要表达于子宫内膜腺上皮的细胞浆;血管内皮生长因子主要表达于子宫内膜腺上皮的细胞浆及部分血管内皮细胞和间质;巨噬细胞移动抑制因子在异位内膜腺上皮细胞表达高于在位内膜及对照组内膜(P<0.05);在位内膜巨噬细胞移动抑制因子的表达在分泌期高于增生期(P<0.05);血管内皮生长因子在异位内膜和在位内膜的表达显著高于对照组内膜(P<0.05);异位内膜巨噬细胞移动抑制因子与血管内皮生长因子表达呈正相关(r=0.630,P<0.001)。结论:异位内膜腺上皮细胞巨噬细胞移动抑制因子及血管内皮生长因子高表达可能相互促使异位内膜血管形成,对子宫内膜异位症的黏附、侵袭、生长起重要作用。

Objective To investigate the expression and association of MIF and VEGF in the endometriosis. Methods Immunohistochemistry method was used to detect the expression of MIF and VEGF protein in 30 cases of ectopic endometrium, 30 cases of eutopic endometrium and 30 cases of endometrium without endometriosis. Results MIF and VEGF protein were expressed in epithelial cells of ecpotic, eutopic and control endometrium. MIF protein was significantly higher in epithelial cells of ecpotic endometrium than in that of eutopic endometrium and control endometrium（P〈0.05）. VEGF protein was significantly higher in epithelial cells of ecpotic endometrium and eutopic endometrium than in that of control endometrium （P〈0. 05）. The expression of MIF in ectopic endometrium epithelial cells had a positive relationship with that of VEGF. Conclusion The overexpressions of MIF and VEGF in epithelial cells of ecpotic endometrium both contribute to angiopoiesis and might be of importance for migratory, adherence, invasion and growth of ectopic endometrium, which may result in endometriosis.