高效液相色谱中同系物保留值收敛现象的研究

Studies on Retention Value Convergent Phenomenon for Homologous Series in High-Performance Liquid-phase Chromatography

在讨论影响溶质保留行为因素的基础上，建立了描述同系物保留值收敛现象的一般模型，对12种同系物（共38组数据）在6种不同组成的二元流动相和8种固定相中的保留值进行了回归分析，相关系数r≥0．974，标准偏差SD≤0．05．绘制了这些同系物的三维保留图形及投影图，并据此对同系物收敛现象及其物理意义进行了讨论，建立了计算收敛点坐标的简便方法．

A general model was established to describe the convergent phenomenon of ho-mologous series on the basis of discussion about the factors affecting solute retention be-haviour. The regression analysis for 12 kinds of homologous series(38 groups of data) was completed in six different mobile phases and on eight stationary phases,respectively.The results indicate that the correlation coefficient r≥0.974, standard deviation SD≥0.05. The surface and the projection of these homologous series' retention values have been drawn in three and two dimension,respectively.Convergent phenomenon and its meaning are also dis-cussed based on these graphs. A simple method for calculating the convergent point coordi-nates is derived by using the first partial derivative.