survivin和caspase-3在病理性瘢痕成纤维细胞中的表达及其意义

Expression and significance of survivin and caspase-3 in fibroblasts from hypertrophy scar and keloid

目的研究病理性瘢痕成纤维细胞中survivin和caspase-3的表达水平,以及两种凋亡相关因子与病理性瘢痕发生、发展机制的关系。方法以普通瘢痕为对照,采用RT-PCR和免疫组化方法,研究体外培养的病理性瘢痕成纤维细胞中survivin和caspase-3mRNA和蛋白的表达变化,并进行比较分析。结果病理性瘢痕成纤维细胞中survivin的mRNA和蛋白(65.47%、72.43%)的表达水平高于普通瘢痕(P<0.01);caspase-3的mRNA和蛋白表达水平低于普通瘢痕(22.85%、6.34%,P<0.01);survivin、cas-pase-3在增生性瘢痕和瘢痕疙瘩中的表达差异有统计学意义(P<0.05)。结论survivin和caspase-3在病理性瘢痕成纤维细胞中的表达异常,在病理性瘢痕的发生、发展中起重要的作用。

To investigate the expression of survivin and easpase- 3 in fibroblasts from hypertrophic sear and keloid and the relationship between their formative and developmental mechanism and these two factors. Methods Using normal sears as a control group, the expression and relation between mRNA and protein of survivin and caspase- 3 in fibroblasts from hypertrophic sear and keloids were evaluated with RT- PCR and immunohistochemical methods. Results The expression of survivin mRNA and protein （65.47 %, 72.43 % ） in pathological scars was higher than that in the control group （P 〈 0.01） ; The expression of easpase- 3 mRNA and protein in pathological scars was lower than that in the control group （22.85% -6.34%, P〈0.01）. The difference in expression of the survivin and easpase - 3 in pathological sear had statistical significance （P 〈 0.05 ）. Conclusion Abnormal expression of survivin and caspase- 3 plays an important role in the formation and development of pathological scars.