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基因物理靶向递送技术

Physical approaches used in gene targeting delivery
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摘要 应用非病毒物理方法进行基因递送已成为细胞内基因递送和基因治疗的基本手段。本文总结目前最常用的非病毒物理方法,重点阐述每种基因递送方法的机制,以及治疗应用中的优缺点。本文还对各种方法的技术特点进行总结,重点阐述了提高递送效率的改进方法,并简要探讨了本领域未来的发展方向。 Gene delivery using nonviral physical approaches has been extensively studied as a basic tool for intracellular gene transfer and gene therapy. This review summarizes the current status of the most commonly used nonviral physical methods, with an emphasis on their mechanism of action for gene delivery, and their advantages and limitations for gene therapy applications. The technical aspects of each delivery system are also reviewed, with a focus on how to achieve optimal delivery efficiency. Future development and further improvement of the current systems are also discussed briefly.
作者 赵应征 鲁翠涛 梅兴国
机构地区 北京军区总医院临床药物研究所 北京麦德姆医学管理集团 军事医学科学院毒物药物研究所
出处 《国际药学研究杂志》 CAS 2008年第1期39-45,共7页 Journal of International Pharmaceutical Research
关键词 基因疗法 基因转移技术 电穿孔法 超声微泡 磁转染法 流体动力法 gene therapy gene transfer techniques electroporation ultrasound microbubbles magneto- fection hydrodynamic delivery
分类号 R346 [医药卫生—基础医学]
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参考文献59

  • 1[1]Liu Y,Yang H,Sakanishi A.Ultrasound:mechanical gene transfer into plant cells by sonoporation[J].Biotechnol Adv,2006,24(1):1-16.
  • 2[2]Heller LC,Ugen K,Heller R.Electroporation for targeted gene transfer[J].Expert Opin Drug Deliv,2005,2(2):255-268.
  • 3[3]Yang NS,Sun WH.Gene gun and other non-viral approaches for cancer gene therapy[J].Nat Med,1995,1(5):481-483.
  • 4[4]Zhao YZ,Luo YK,Liang HD,et al.Comparing transfection efficiency and safety for antisense oligodeoxyribonucleotide between phospholipids-based microbubbles and liposomes[J].J Drug Target,2006,14(10):687-693.
  • 5[5]Liu F,Song Y,Liu D.Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA[J].Gene Ther,1999,6(7):1258-1266.
  • 6[6]Scherer F,Anton M,Schillinger U,et al.Magnetofection:enhancing and targeting gene delivery by magnetic force in vitro and in vivo[J].Gene Ther,2002,9(2):102-109.
  • 7[7]Nishikawa M,Huang L.Nonviral vectors in the new millennium:delivery barriers in gene transfer[J].Hum Gene Ther,2001,12(8):861-870.
  • 8[8]Schughart K,Rasmussen UB.Solvoplex synthetic vector for intrapulmonary gene delivery.Preparation and use[J].Methods Mol Med,2002,69:83-94.
  • 9[9]Desigaux L,Gourden C,Bello-Roufai M,et al.Nonionic amphiphilic block copolymers promote gene transfer to the lung[J].Hum Gene Ther,2005,16:821-829.
  • 10[10]Freeman DJ,Niven RW.The influence of sodium glycocholate and other additives on the in vivo transfection of plasmid DNA in the lungs[J].Pharm Res,1996,13(2):202-209.

二级参考文献37

  • 1Yang PL,Althage A,Chung J,Chisari FV.Hydrodynamic injection of viral DNA:a mouse model of acute hepatitis B virus infection.Proc Natl Acad Sci USA 2002; 99:13825-13830
  • 2Yokoi H,Mukoyama M,Nagae T,Mori K,Suganami T,Sawai K,Yoshioka T,Koshikawa M,Nishida T,Takigawa M,Sugawara A,Nakao K.Reduction in connective tissue growth factor by antisense treatment ameliorates renal tubulointerstitial fibrosis.J Am Soc Nephrol 2004; 15:1430-1440
  • 3Chang J,Sigal LJ,Lerro A,Taylor J.Replication of the human hepatitis delta virus genome Is initiated in mouse hepatocytes following intravenous injection of naked DNA or RNA sequences.J Virol 2001;75:3469-3473
  • 4Kobayashi N,Kuramoto T,Yamaoka K,Hashida M,Takakura Y.Hepatic uptake and gene expression mechanisms following intravenous administration of plasmid DNA by conventional and hydrodynamics-based procedures.J Pharmacol Exp Ther 2001;297:853-860
  • 5Hofman CR,Dileo JP,Li Z,Li S,Huang L.Efficient in vivo gene transfer by PCR amplified fragment with reduced inflammatory activity.Gene Ther 2001; 8:71-74
  • 6Kobayashi N,Matsui Y,Kawase A,Hirata K,Miyagishi M,Taira K,Nishikawa M,Takakura Y.Vectorbased in vivo RNA interference:dose-and timedependent suppression of transgene expression.J Pharmacol Exp Ther 2004; 308:688-693
  • 7Matsui Y,Kobayashi N,Nishikawa M,Takakura Y.Sequence-specific suppression of mdr1a/1b expression in mice via RNA interference.Pharm Res 2005;22:2091-2098
  • 8Takahashi Y,Nishikawa M,Kobayashi N,Takakura Y.Gene silencing in primary and metastatic tumors by small interfering RNA delivery in mice:quantitative analysis using melanoma cells expressing firefly and sea pansy luciferases.J Control Release 2005; 105:332-343
  • 9Wang G,Tschoi M,Spolski R,Lou Y,Ozaki K,Feng C,Kim G,Leonard WJ,Hwu P.In vivo antitumor activity of interleukin 21 mediated by natural killer cells.Cancer Res 2003; 63:9016-9022
  • 10Kobayashi N,Kuramoto T,Chen S,Watanabe Y,Takakura Y.Therapeutic effect of intravenous interferon gene delivery with naked plasmid DNA in murine metastasis models.Mol Ther 2002; 6:737-744

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国际药学研究杂志
2008年 第1期

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