葛根素对炎症反应综合征大鼠的治疗作用

Effect of puerarin to cure the rats with systemic inflammatory response syndrome

目的建立全身炎症反应综合征（SIRS）大鼠模型，探讨葛根素治疗实验性SIRS的效果及其机制。方法采用腹腔注射酵母多糖．石蜡悬液（ZPS）制备SD大鼠SIRS模型。通过SIRS大鼠静脉注射葛根素后动物死亡率变化，了解葛根素治疗实验性SIRS的效果。采用ELISA定量检测试剂盒了解葛根素对SIRS大鼠外周血中TNF-α、IL-6和IL-10水平的调控作用。采用单因素方差分析和x^2检验对实验数据进行统计学分析。结果750mg／kg和1000mg／kg酵母多糖ZPS均可诱导大鼠发生SIRS。腹腔注射1000mg／kg酵母多糖ZPS的大鼠死亡率为75．0％，静脉注射62．5mg／kg葛根素后死亡率可降至16．7％（P〈0．01）。大鼠腹腔注射750mg／kg酵母多糖ZPS后24h，外周血中TNF-α、IL-6和IL-10浓度分别为（30．87±6．81）pg／ml、（525．20±92．45）pg／ml和（1．37±0．17）ng／ml，静脉注射62．5mg／kg葛根素后TNF-α、IL-6和IL-10浓度分别为（16．71±3．75）pg／ml、（399．30±77．87）pg／ml和（1．95±0．17）ng／ml。结论本研究建立了稳定的SIRS大鼠模型。葛根素对实验性SIRS有良好疗效，下调促炎因子TNF-α和IL-6水平，上调抗炎因子IL-10水平可能是葛根素抗SIRS的作用机制之一。

Objective To study the effects of puerarin eherbal preparation on experimental systemic inflammatory response syndrome （SIRS） in rat model. Method A SIRS animal model in SD rat was established by intraperitoneal injection with zymosan-parallm suspension （ZPS）. According to the mortality of SIRS rats treated with puerarin, the effects of puerarin to cure experimental SIRS was determined. By using commercial Quantitative Detection ELISA Kits, the levels puerarin blood TNF-α, IL-6 and IL-10 of SIRS rats treated wiht puerarin were assayed . The experimental data were statistically analyzed using one-way ANOVA analysis and x^2 tests. Results The ZPS containing 750 mg/kg or 1000 mg/kg zymesan could be enough to induce SIRS in rats. 75.0% rats induced with ZPS containing 1000 mg/kg zymosan intraperitoneally injected died and the mortality could be decreased to 16.7% after puerarin 62.5 mg/kgintravenously injected （ P 〈 0.01 ）. Twenty-four hours after intraperitoneal injection of ZPS containing 750 mg/kg zymosan, the blood levels of TNF-α, IL-6 and IL-10 were （30.87±6.81） pg/ml, （525.20±92.45） pg/ml and （1.37±0.17） ng/ml, respectively. When 62.5 mg/kg puerarin was administed intrasrenously into animals induced by zymosan, theblood levels of TNF-α, IL-6 and IL-10 could be decreased to （ 16.71 ± 3.75） pg/ml, （399.30 ± 77.87） pg/ml and （ 1.95 ± 0.17） ng/ml, respectively （P 〈 0.01）. Coclusions Puerarin has therapeutic effects on experimental SIRS in which the down-regulation of inflammation-promoting factors, TNF-α and IL-6, levels and up-regulation of inflammation-inhibiting factor, IL- 10, level may be one of the mechanisms of puerarin against SIRS.