补骨脂多糖的提取及免疫活性的研究

Study on Immunity Activity of Polysaccharide Extracted from Psoralea Corylifolia.L

目的:从补骨脂中提取出多糖,并对其免疫活性进行初步研究。方法:采用水提醇沉法提取补骨脂多糖,Sevage法除蛋白,苯酚-硫酸分光光度法测定多糖的含量。用绵羊红细胞(SRBC)为抗原给小鼠注射后,将补骨脂多糖灌服小鼠7 d,加强免疫后,检测血清中细胞因子γ-干扰素(IFN-γ)和白细胞介素-2(IL-2)的生成水平,评价其免疫活性。结果:补骨脂多糖的含量为3.84%,平均回收率97.31%,RSD为1.90%,线性范围为5～40μg·ml^(-1)。实验组的IFN-γ和IL-2激发水平均显著高于对照组(P<0.05)。结论:补骨脂多糖对正常小鼠机体免疫有增强作用。

Objective： To extract polysaccharide of Psoralea Corylifolia. L and to study its immunity activity. Method. Polysacchayide extracted from Psoralea Corylifolia. L by water-extraction-alcohol-precipitation method was determined by phenol sulfonic acid spectrophotometry. SRBC was employed as antigens to be injected to mice, after the secondary immunization, the level of the concentration of serum IFN-γ and IL-2 were determined. Result： The contents of polysaccharide of samples was 3.84%, the average recovery was 97.31% , standard curve was liner in the concentration range 5 -40 μg· ml^-1 Cytokines （IFN-γ and IL-2 ） of Psoralea Corylifolia. L polysaccharide were all significantly higher than those of control groups. Conclusion： Psoralea Corylifolia. L polysaccharide could enhance the immune function in normal mice.