HPLC法测定补气养血汤中阿魏酸的含量

Determination of Ferulic Acid in the Soup for Replenishing Qi and Raising Blood by HPLC

目的:建立补气养血汤中阿魏酸的含量测定方法。方法:采用高效液相色谱法,使用Nova-paKC_(18)(150 mm×3.9 mm,5μm)柱,以甲醇-1%乙酸(30:70)为流动相,检测波长320 nm。结果:在0.2～1.0μg范围内,含量与峰面积线性关系良好,阿魏酸的平均回收率为100.0%,RSD为1.97%,补气养血汤中阿魏酸的含量不少于0.1503 mg·ml^(-1)。结论:该方法简便,灵敏度高,重现性好,可用于补气养血汤的初步质量控制。

Objective： To establish a method for determination of ferulic acid in the Soup for replenishing Qi and Raising Blood. Method： Nova-paK C18 column（ 150 mm× 3.9 ram,5μm） was used,the mobile phase was methanol-1% acetic acid （30： 70） , detection wavelength was at 320 nm. Result： The linear range was 0.2 - 1.0 μg. The average recovery of Ferulic Acid was 100.0% （RSD = 1.97% , n = 5 ）. The content of Ferulic Acid in the Soup for Replenishing Qi and raising Blood was no less than 0. 1503 mg·ml^-1. Conclusion： The method is simple,accurate and can be used to control the quality of the soup for replenishing Qi and raising Blood.