中国汉族男性军人ADRA2A基因多态性研究

An experimental study on ADRA2A gene polymorphism of the servicemen of Han ethnic in PLA

目的探讨中国汉族男性军人α2A肾上腺素能受体基因(ADRA2A)的遗传多态性及其与有氧耐力素质的关联性。方法108名无训练史的汉族健康男性军人进行18周的有氧耐力训练,测试训练后5000m跑的成绩。DNA测序分析ADRA2A基因的G6412C、T6623A、C6645C三个位点的多态性。分析5000m跑成绩与基因多态性之间的关系。结果3个多态位点的基因分布频率均符合Hardy-Weinberg平衡。ADRA2A基因6623位点杂合子A/G基因型频率为50.0%,高于纯合子G/G基因型(26.0%)和A/A基因型(24.0%);G等位基因频率为49.0%,低于A等位基因频率(51.0%)。6623位点基因型为A/A的汉族男性军人5000m测试成绩(18.97±0.65min)明显快于基因型为A/G者(20.95±0.82min),差异有显著性意义(P<0.05),G/G基因型5000m测试成绩(20.21±0.73min)与A/G、A/A基因型比较无显著性差异(P>0.05)。未发现G6412C、C6645C单点多态性与有氧耐力素质有明显的相关性。结论ADRA2A基因位点T6623A多态性可作为中国汉族男性青年个体有氧耐力素质的基因标记,而G6412C和C6645C多态性不是预测个体有氧耐力素质的基因标记。

Objective To explore the features of polymorphism in ADRA2A gene phenotypes and their relationship to oxygen endurance in the Han servicemen in PLA.Methods One hundred and eight healthy recruits of Han ethnic were subjected to a 5000-meter race program for 18 weeks,and then the tests were done after a 5000-meter race.The ADRA2A allelic variant and genotype were analyzed by polymerase chain reaction and sequencing,and the features of polymorphism in three sites,G6412C,T6623A and C6645C,were determined with biochemical technique.The relationship between the results after the 5000-meter race,and the polymorphism of ADRA2A was then analyzed.Results The genotype distribution at all the three locations was consistent with the Hardy-Weinberg equilibrium.The genotype frequency in T6623A of A/G（50%）was higher than that of G/G（26%）and A/A（24%）.The allele frequency of A（51%）was higher than that of G（49%）.The time for completing 5000-meter running was significantly longer in recruits carrying A/G（20.95±0.82min）than those carrying A/A（18.97±0.65min,P〈0.05）.The time to complete 5000-meter running in recruits carrying GG（20.21±0.73min）had no significant difference compared with those carrying A/G and A/A（P〉0.05）.There was no significant association between the oxygen endurance capacity and the polymorphism of G6412C and C6645C.Conclusion The T6623A of ADRA2A was the ideal gene marker for predicting endurance capacity,but G6412C and C6645C polymorphism is not ideal gene marker for oxygen endurance.