葛根素对高脂饮食大鼠胰岛素敏感性的影响

Influence of insulin sensitivity on insulin resistance rats treated by puerarin

目的观察葛根素（puerarin,pue）对高脂饮食诱导的胰岛素抵抗（insulin resistance,IR）大鼠胰岛素敏感性的影响。方法以尾静脉注射小剂量链脲菌素（streptozotocin,STZ）和高脂饲料喂养诱导IR大鼠模型,给药治疗8wk后,采用改良的高血浆胰岛素-正常血糖钳夹技术,评价葛根素对大鼠胰岛素敏感性的影响。结果高脂饲料喂养诱导IR大鼠基础血糖值（BBG）、基础血浆胰岛素（BINS）、稳态血浆胰岛素（SINS）均较正常组明显增高,而葛根素治疗组BBG、BINS、SINS较IR模型组均明显降低;高血浆胰岛素-正常血糖钳夹试验中60-120min的平均葡萄糖输注率（GIR60-120）,IR模型组明显低于正常组,而葛根素高、中剂量组较IR模型组升高。结论葛根素可降低实验性IR大鼠的血糖和血浆INS水平,改善机体对胰岛素的敏感性。

Aim To investigate the effect of puerarin （Pue） on the experimental insulin resistance （IR） and the mechanism of Pue-treated metabolic syndrome （MS）. Methods IR rat model was induced by i. v.small dosage streptozotocin （STZ） and high fatty diet. The effect of Pue on insulin sensitivity was studied by reformed hyperinsulinism euglycemia clamp technique （HEPC） on IR rats. Results The basic blood glucose （BBG）, basic blood plasma insulin （BINS） and stable basic blood plasma insulin （SINS） of IR rats induced by high-fat feed were higher than those of control group, and those of the group treated by Pue were observably lower than those of IR model group. In HECT text,the average rate of glucose injection of IR model group was lower than that of control group from 60 min to 120 min, but the rate of high dose and middle dose of Pue group was significantly higher than that of IR model group. Conclusion The results suggest that Pue can improve the insulin sensitivity, and reduce basic blood glucose and blood plasma insulin of the experimental insulin resistance rats.