HPLC测定番泻叶中5种主要化学成分的含量

Determination of five primary chemical constituents in Cassia angustifolia by HPLC

目的：建立番泻叶中主要化学成分的含量测定方法。方法：采用HPLC，DiamonsilC18柱（4.6mm×250mm，5μm），流动相乙腈-1％冰醋酸梯度洗脱（10：90-15：85-18：82-20：80-25：75），流速1mL·min^-1，柱温40℃，检测波长270nm。结果：5种化学成分均达到基线分离，各成分都有较宽的线性范围和良好的线性关系。结论：本法快速、灵敏、准确，可靠，重复性好，可用于中药番泻叶药材的质量控制。

Objective： To establish a method for determining the content of primary chemical constituents in the leaves of Cassia angustifolia. Method： The HPLC with Diamonsil Cl8 （4.6 mm×250mm, 5 μm）column was used , acetonitrile-1% acetic acid （10： 90-15： 85-18： 82-20： 80-25： 75） in a gradient manner was used as a mobile phase, with flow rate of 11mL·min^-1, column tem- perature at 40℃ and detection wavelength at 270 nm. Result： The results showed that 5 effective components all separated well and showed good linearity. Conclusion： The method was proved to be rapid, sensitive, accurate, credible and repeatable. It can be applied to quality control of Folium Sennae.