骨髓增生异常综合征线粒体呼吸链酶复合体的变化

The change of mitochondrial respiratory chain complex in patients with myelodysplastic syndrome

目的:研究骨髓增生异常综合征(MDS)患者骨髓单个核细胞线粒体呼吸链的功能变化并分析其与MDS的关系。方法:测定26例MDS患者与10例骨髓象正常者的单个核细胞线粒体呼吸链酶复合体Ⅰ、Ⅲ、Ⅳ的活性。结果:MDS患者呼吸链酶复合体Ⅰ、Ⅲ、Ⅳ的活性明显低于对照组(P<0.05);线粒体呼吸链酶复合体Ⅰ、Ⅲ的活性在RAEB-Ⅰ、RAEB-Ⅱ组与RA、RARS组的差异无统计学意义(均P>0.05),而酶复合体Ⅳ的活性在RAEB-Ⅰ、RAEB-Ⅱ组较RA、RARS组高,差异有统计学意义(P<0.05)。结论:线粒体呼吸链酶复合体的活性在MDS患者中降低,可能与MDS的病态造血及无效造血有关。

Objective：To investigate the function of mitochondrial respiratory chain in bone marrow mononuclear eell（BMMNC） of myelodysplastie syndrome（MDS） and its relationship with MDS. Method： Activities of respiratory chain complexes Ⅰ , Ⅱand Ⅳwere examined in BMMNC from 26 patients with MDS and 10 normal controls. Result：Activities of respiratory chain complexes Ⅰ , Ⅱand Ⅳ in BMMNC were significantly lower in MDS patients than that in normal controls（ P 〈0.05）. According to WHO classification,activities of respiratory chain complexes Ⅰ , Ⅲand Ⅳin BMMNC were significantly lower in MDS RAEB-Ⅰ , RAEB-Ⅱ and RA, RARS than normal controls（ P d0.05）. Activities of respiratory chain complexes Ⅰ and Ⅲin type RAEB- Ⅰ ,RAEB-Ⅱ and type RA,RARS showed no significance difference（ P 〉0.05）, but the activities,of respiratory chain complexes Ⅳwas significantly higher in MDS RAEB- Ⅰ , RAEB-Ⅱ than RA, RARS（ P 〈0.05）. Conclusion： Reduced activities of respiratory chain complexes may be related to dysplastie hematopoiesis and ineffective hematopoiesis.