摘要
目的:探讨胃癌及癌前病变中微卫星不稳定(MSI)的变化.方法:饱和氯化钠法提取组织DNA,PCR-SSCP变性聚丙烯酰胺凝胶电泳检测MSI,根据规定标准来判定microsatellite stable(MSS),low frequency MSI(MSI-L)和high-frequency MSI(MSI-H),计算总MSI率.结果:MSI在萎缩性胃炎,肠上皮化生,不典型增生,早期胃癌和进展期胃癌中检出率分别为13.3%、16.7%、23.3%、30%和40%,三种癌前期病变和早期胃癌之间有显著差异(χ^2值分别为4.364、2.522、3.089,P〈0.05),早期胃癌与进展期胃癌没有显著差异(χ^2值为0.071 P〉0.05),高分化腺癌中MSI的阳性率明显高于低分化腺癌(χ^2值为4.022,P〈0.05).结论:MSI是胃癌发生中的早期分子标志,其阳性的胃癌恶性程度相对较低.
AIM: To investigate the changes in microsatellite instability (MSI) in gastric cancer and pre cancerous lesions.
METHODS: DNA was extracted by the method of saturated sodium chloride. MSI was detected by PCR-SSCP denaturing PAGE and silver staining, microsateUite stability (MSS), low-frequency MSI (MSI-L) and high-frequency MSI (MSI-H) were evaluated following the international MSI workshop-recommended standard.
RESULTS: According to the international MSI workshop recommended standard, the total positive rate of MSI was 13.3 % for atrophic gastritis, 16.7% for intestinal metaplasia, 23.3% for atypical hyperplasia, 30% for early stage gastric cancer, and 40% for advanced stage gastric cancer. There were differences between three kinds of precancerous lesions and gastric cancer (χ^2 was 4.364, 2.522 and 3.089, respectively, P 〈 0.05). There was no difference between early and advanced stage gastric cancer (χ^2 was 0.071). The positive rate of MSI in well-differentiated adenocarcinoma was higher than that in poorly differentiated adenocarcinoma (χ^2 was 4.022, P 〈 0.05).
CONCLUSION: MSI is an early molecular marker in the pathogenesis of gastric cancer. The malignant degree of MSI positive gastric cancer may be low.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第1期94-97,共4页
World Chinese Journal of Digestology
基金
辽宁省自然科学基金
No.2006225007-4~~
关键词
胃癌
微卫星不稳定
饱和氯化钠法
Gastric cancer
Microstaellite instability
Method of staturated sodium chloride