热处理小鼠H_(22)肝癌细胞的抗肿瘤作用

Antitumor Effects of Heat-treated Mice H 22 Hepatic Carcinoma Cells in Mice

目的：比较肿瘤细胞体外经不同温度时间热处理后的抗肿瘤效应。方法：采用普通水浴加热方法将小鼠Ｈ２２肝癌细胞经一定温度时间处理后制成瘤苗，免疫ＩＣＲ小鼠，然后腹腔移植Ｈ２２细胞，观察小鼠的平均存活天数，免疫小鼠脾细胞体外对Ｈ２２肝癌细胞的杀伤作用。结果：６５℃／３０分钟水浴加热处理能完全杀灭Ｈ２２细胞；６５℃／３０分钟热处理制备的瘤苗能显著延长荷瘤小鼠的存活时间（Ｐ＜０．０１）；并且该瘤苗免疫小鼠的脾细胞杀伤Ｈ２２细胞作用显著高于荷瘤小鼠及正常小鼠（Ｐ＜０．０１）。结论：采用水浴加热方法制备的肿瘤瘤苗能增强机体的抗肿瘤作用，６５℃／３０分钟为水浴方法制备瘤苗的适宜条件。

Objective: To compare in vitro antitumor effect of H 22 tumor vaccine prepared by different temperature of heat. Methods:By warming of common water bath,H 22 hepatic carcinoma cells from mice were prepared as vaccine. Then ICR mice were immunized by intraperitoneal transplantation of H 22 cells. The mean survival day of the mice and in vitro killing effect of spleen cells to H 22 hepatic carcinoma cells were observed. Results: The H 22 tumor cells were completely killed by treatment of 65 ℃ water bath for 30 minutes. The mean survival time of tumor -bearing mice immunized with tumor vaccine by treatment of 65℃ water bath for 30 minutes markedly prolonged compared with control (P< 0.01 ). The cytotoxicity of spleen cells of mice immunized by the tumor vaccines was statistically higher than those of normal or tumor-bearing mice. Conclusions: Heat-treated tumor cells in water bath have capacity of enhancing antitumor immunity and treatment of 65 ℃ water bath for 30 minutes is an optimal method for preparing tumor vaccine.