不同参数低频电磁场刺激对人胚肌腱细胞体外增殖的影响

Various Parameters of Low-frequecy Electromagnetic Stimulation on Cultured Human Embryo Tenocytes Proliferation.

目的：探讨低频电磁场促进人胚肌腱细胞增殖的最佳物理参数。方法：应用正交设计方案，比较不同参数（频率、波形、场强）组合的电磁场刺激对人胚肌腱细胞体外增殖的影响。并对电磁场波形进行Ｆｏｕｒｉｅｒ变换，分析其频谱组成与效应的关系。结果：这些电磁场均促进肌腱细胞增殖（Ｐ＜０．０１），其中１．７Ｈｚ、５～６×１０－４Ｔ正弦波电磁场效果最佳（Ｐ＜０．０５），肌腱细胞可能对低频成分更敏感。结论：为临床应用电磁场促进肌腱愈合提供了选择物理参数的依据。

Objective: To explore the optimal parameters of low frequency electromagnetic fields (LFEMF) stimulating human embryo tenocytes proliferation. Methods: Referring to Orthogonal design, the effects of LFEMF with various frequencies, flux densities and waveforms on tenocytes growth in vitro were compared,then the waveforms were analysed with Fourier transforms. Results: All the parameters of LFEMF promoted human embryo tenocytes proliferation(P< 0.01 ), 1.7 Hz 、5～6×10 -4 T sinusoidal electromagnetic field had the best response(P< 0.05 ). Human embryo tenocytes were more sensitive to low-frequency components.Conclusions: This study provides a scientific parameter selection basis of LFEMF for clinical tendon healing.