甲醛染毒大鼠脂质过氧化水平分析

STUDY ON LIPID PEROXIDE IN THE BLOOD AND LIVER OF FORMALDEHYDE TREATED RATS

用不同剂量（5，10，20，50，100mg／kg）甲醛经腹腔注射染毒SD大鼠后24小时，以及10mg／kg染毒后2，6，24小时收集大鼠血和肝脏。对样品的测定结果显示，红细胞SOD活性，全血GSH－PX活性，红细胞内谷胱甘肽含量以及血浆与肝组织MDA浓度等五项指标与染毒剂量均有不同程度相关，其中红细胞 SOD对甲醛最为敏感，5mg／kg时就比对照组有明显降低，在时间效应关系中可以见到红细胞内SOD活性和全血GSH－PX活性染毒（10mg／kg）后24小时内随时间延长而逐渐降低。染毒后2小时血浆中MDA浓度最高，红细胞内GSH含量最低，两者在染毒后24小时基本上恢复到对照组水平。结果提示，甲醛可以启动体内脂质过氧化反应，损伤自由基和活性氧的清除系统。

The blood and livers of rats were collected 24 hrs. after i. p. administration of formaldehyde (5 ) 10,, 20, 50 mg/kg ). The activity of blood glutathione peroxidase (GSH-PX),superoxide dismutase (SOD) activities, glutathione conte nt in erythrocyte and the concentration ofmalondialdehyde (MDA) in plasma and liver were investigated. The results showed that therewas a significant relationship between the four indexes and the dosages. SOD in erythrocytewas the most susceptible to formaldehyde. The content of GSH in erythrocyte and MDA inplasma changed significantly compared with those of controls when treated with formaldehyde(10mg/kg) 2 hrs. later. They resumed to the level of controls 24 hrs. after the treatment. Theactivity of SOD and GSH-PX became lower and lower at 2, 6, 24 hrs. after the treatment. Thisexperiment revealed that formaldehyde might initiate lipid peroxide,which in turn would damage the activity of SOD and GSH-PX and reduce the content of GSH in erythrocyte.