SDS-PAGE电泳测定乳清蛋白方法的研究

Determination of α-lactalbumin and β-lactoglobulin in whey protein by SDS-PAGE electrophoresis

实验建立扫描定量测定乳清蛋白中α-乳白蛋白和β-乳球蛋白含量的SDS-PAGE电泳分析方法。外标法定量,α-乳白蛋白和β-乳球蛋白两种组分线性范围分别为0.25～1.75μg和0.16～1.44μg,线性关系良好,相关系数分别为0.9940和0.9912。加标回收率分别为88.6%～86.3%,RSD分别为3.8%～4.2%。对乳清粉和奶粉的分析结果表明,SDS-PAGE电泳分析方法是一种简易、快速、准确测定α-乳白蛋白和β-乳球蛋白含量的方法。

A method was established for the determination of α-lactalbumin and β-lactoglobulin by SDS-PAGE electrophoresis. The quantified analysis of external standard calibration curves was used. The linear range of α-lactalbumin and β-lactoglobulin were respectively 0.25-1.75 μg and 0.16-1.44 μg. There were good linear correlations about the two components. The correlation coefficients were 0.9940 and 0.9912. The average recoveries were 88.6%-86.3% with RSD 3.8%-4.2%. This method was simple and fast, it is a reliable means to measure the content of α-lactalbumin and β-lactoglobulin,