盾叶薯蓣提取薯蓣皂甙元后废料发酵制取酒精的研究

Research on Fermentation Conditions for Alcohol Production by Residue from Diosgenin Processing

为研究盾叶薯蓣提取薯蓣皂甙元后废料发酵制取酒精的最佳实验条件,以酒精产量为评价参数,采用五因素四水平的正交试验,用可见分光光度法对16种制取方法所得到的酒精进行比较分析。结果表明,酒精与重铬酸钾溶液经加热显色后,在585nm波长有最大吸收波长,酒精体积百分比浓度在0.028%～0.24%范围内线性关系良好(r=0.9998);发酵时间对于酒精产量影响最大,在实验室条件下,采用50g原料使用7U/g原料淀粉酶、110U/g原料纤维素酶、110U/g原料糖化酶、0.04g/g原料酵母发酵4d,可使酒精产量达到最大。

To research the optimum fermentation conditions for alcohol production by residue from diosgenin processing, several important factors were analyzed in the orthogonal test of five factors and four levels. The content of alcohol was valued as an evaluation index using spectrophotometry. The results indicated that the linear range of calibration curve was 0.028%～0.24% （r=0.9998） after color reaction, and optimal fermentation conditions were determined as follows： the use level of α- amylase as 7 U/g, cellulase 110 U/g, glucoamylase 110 U/g, yeast 0.04 g/g, and 4 days fermentation time.