草莓花药组织培养脱毒快繁技术研究

Study on the Virus-free and Rapid Propagation in Tissue Culture of Strawberry Anther

[目的]研究草莓花药组织培养的脱毒快繁技术,实现草莓无病毒栽培。[方法]在基本培养基中分别添加0.5、1.0、1.5、2.0mg/L的6-BA和KT,研究不同激素浓度对不同发育期的草莓花药愈伤组织诱导和不定芽的诱导、增殖的影响。[结果]单核期花药的诱导率高达78.9%,单核靠边期或接近双核期花药的诱导率只有21.1%。6-BA和KT为2.0mg/L时,愈伤组织的生长状况均最好,细胞间联系致密,表面突起更多、更紧。草莓花药在6-BA中比KT分化出的愈伤组织多。6-BA1.5mg/L的培养基愈伤组织分化率最高,诱导丛芽的效果最好,丛芽多而健壮,丛芽发生率达112.6%,增殖系数达1.95。[结论]6-BA比KT更适合诱导草莓花药愈伤组织。草莓花药愈伤组织的诱导、不定芽的诱导和增殖最适合的培养基均是MS+NAA0.2mg/L+6-BA1.5mg/L。

[Objective] The purpose of this research was to study the virus-free and rapid propagation technology in tissue culture of strawberry anther so as to realize the virus-free cultivation of strawberry.[Method] 0.5, 1.0, 1.5 and 2.0 mg/L 6-BA and KT were added into basic media to study the effects of different hprmones concentrations on the callus induction and adventitious bud induction and multiplication of strawberry anthers in different development periods.[Resuh] The induction rate of anther in the uninucleate stage was up to 78.9 % and that in the late uninucleate stage or in the period close to binucleate stage was only 21.1%. When the 6-BA and KT concentrations were 2.0 mg/L, the growth status of callus was best and the cells contacted with each other compactly with more and denser surface protuberances. Strawberry anther in 6-BA differentiated out more callus than that in KT. The differentiation rate of callus on the medium with 1.5 mg/L 6-BA was highest with the best effect of inducing clumpy buds with many and hale clumpy buds and the occurrence rate reaching 112.6 %, and the multiplication coefficient reached 1.95.[Conclusion] 6-BA was more suitable to induce callus of strawberry anthers than KT. The optimum medium for both callus induction and adventitious bud induction and multiplication of strawberry anthers was MS ＋ NAA 0.2 mg/L ＋ 6-BA 1.5 mg/L.