4种芦蒿的鉴定·分类与评价

Identification,Classification and Evaluation of 4 Varieties of Artemisia selengensis

[目的]为研究芦蒿的种质资源和遗传机制奠定基础。[方法]以在南京地区栽培的4个芦蒿品种(红芦蒿、伏秋青芦蒿、冬春青芦蒿和白芦蒿)为材料,利用形态学观察和同工酶技术对4个芦蒿品种进行鉴定与分类,并对其农艺性状进行综合评价。[结果]通过形态学观察,可初步确定4份材料为芦蒿,且存在明显的形态差异。4个芦蒿品种有12条过氧化物酶共同谱带和6条酯酶共同谱带,分别占总位点数的92.3%和50.0%,从酶蛋白水平上进一步证实4份材料均属芦蒿种。4份材料间同工酶谱带也有显著差异,与表型差异相一致。田间调查表明,在相同生长环境中伏秋青芦蒿和冬春青芦蒿的生长势较好、产量较高,这与同工酶分析结果相一致。[结论]伏秋青芦蒿和冬春青芦蒿在芦蒿育种中具有较高的利用价值。

[Objective] The research aimed to lay the foundation for studying the germplasm resource and the genetic mechanism of A rtemisia selengensis. [Method] With 4 varieties ofA. selengensis （Red A. selengensis, Green A. selengensis in summer and autumn, Green A. selengensis in winter and spring, White A. selengensis ） cultivated in Nanjing area as tested materials, 4 varieties of A. selengensis were identified and classified by morphological observation and isozyme technology. And their agronomic traits were comprehensively evaluated. [Result] Through morphological observation, 4 materials were preliminarily confirmed as A. selengensis and they had obvious morphological differences. There were 12 common peroxidase bands and 6 common esterase bands among 4 varieties of A. selengensis, being 92.3 % and 50.0 % of total site number respectively,which further proved that 4 materials belonged to A, selengensis at the zymoprotein level, The isozyme bands also had significant differences among 4 materials, which were in accordance with the phenotypic differences, The field investigation showed that Green A. selengensis in summer and autumn, Green A.selengensis winter and spring had better growth vigor and higher yield in the same growth environment, which was in accordance with the results of isozyme analysis. [Conclusion] Green A. selengensis in summer and autumn, Green A. selengensis in winter and spring had higher utilization values in the breeding of A, selengensis.