摘要
[目的]提高红海榄抗盐胁迫的能力。[方法]用添加5、15、25mmol/LCaCl2的培养基培养盐胁迫下的红海榄幼苗,研究钙对盐胁迫下红海榄幼苗生长及膜质过氧化的影响。[结果]根及叶片的鲜重在5mmol/L钙处理时明显高于对照和单独盐处理,15mmol/L钙处理达到峰值。5mmol/LCa^2+处理根的SOD活性一直维持较高水平而达到最大值,15mmol/LCa^2+处理叶片的SOD活性最大,4个月后25mmol/LCa^2+处理的最大,比对照和盐处理分别高28.49%和23.32%。5mmol/LCa^2+处理根的MDA含量一直雏持较低水平而达到最小值。15mmol/LCa^2+处理叶片的MDA含量最低,2个月后明显低于其他组,仅为单独盐处理的46、98%。钙能有效提高红海榄幼苗根及叶片的鲜重和SOD活性,降低丙二醛含量,提高其耐盐性。[结论]5~15mmol/LCa^2+有效提高了红海榄幼苗抗盐胁迫的能力。
[Objective] The study aimed to enhance the resistance capability of Rhizophora stylosa to salt stress. [Method] The R. stylosa seedlings under salt stress was cultured on the medium added 5, 15 and 25 mmol/L CaCl2 to study the effect of Ca^2+ on the growth and lipid peroxidatiou of R. stylosa seedlings under salt stress. [Result] The fresh weights of root and leaf treated with 5 mmol/L Ca^2+ were obviously higher than that in CK and the alone salt treatment, and reached peak value in the treatment with 15 mmol/L Ca^2+ . SOD activity in root treated with 5 mmol/L Ca^2+ was always sustained at higher level and reached maximum, that in leaf treated with 15 mmol/L Ca^2+ was highest and that in leaf treated with 25 mmol/L Ca^2+ was highest after 4 months, being 28.49% and 23.32% higher than that in CK and salt treatment resp. MDA content in root treated with 5 mmol/L Ca^2+ was always sustained at lower level and reached minimum, and that in leaf treated with 15 mmol/L Ca^2+ was lowest and obviously lower than that in the other groups after 2 months, being only 46.98% of that in alone salt treatment. Ca^2+ could enhance the fresh weight and SOD activity of root and leaf of R. stylosa seedlings effectively, reduce MDA content and enhance salt resistance of R. stylosa seedlings. [Conclusion] Ca^2+ in 5 - 15 mmol/L enhanced the resistance capability of R. stylosa seedlings to salt stress effectively.
出处
《安徽农业科学》
CAS
北大核心
2008年第3期879-880,910,共3页
Journal of Anhui Agricultural Sciences
基金
广西师范大学博士点建设项目(XKY2006ZD02)
关键词
钙
盐胁迫
红海榄
超氧化物歧化酶
膜脂过氧化
Calcium
Sah stress
Rhizophora stylosa
Superoxide Dismutase
Lipid peroxidation