海南龙血树离体快繁的研究

Study on Rapid Propagation in vitro of Dracaena cambodiana

[目的]加强龙血树组织培养的再生体系,为其迅速繁殖及大规模工厂化育苗提供依据。[方法]以龙血树的幼嫩茎段为外植体,用9种6-BA、NAA不同浓度配比的诱导培养基和NAA不同浓度的生根培养基进行对比试验,研究海南龙血树的组织培养与快速繁殖。[结果]龙血树幼嫩茎段的诱导和生长与6-BA、NAA有关系,两者的配比直接影响茎段的再生频率。6-BA浓度对愈伤组织的形成影响极为显著,NAA的影响不是很明显。MS+0.3 mg/L NAA的生根时间最短,15 d左右生根,根数多,根系良好,移栽成活率高达95%。[结论]龙血树幼嫩茎段的诱导和不定芽增殖的最适培养基为MS+6.0 mg/L 6-BA+0.5 mg/L NAA,增殖系数达10以上,最适宜的生根培养基为MS+0.3 mg/L NAA。

[Objective] The research aimed to strengthen the tissue culture regeneration system of Dracaena cambodiana and provide basis for its rapid propagation and industrial seedling on a large scale. [Method] With tender stem segments of Dracaena cambodiana as explants,comparative test was conducted on 9 kinds of induction medium with different concentrations of 6-BA and NAA,and rooting medium with different concentrations of NAA to study the tissue culture and rapid propagation of Dracaena cambodian. [ Result] Tne induction and growth of tender stem segment of D. cambodliana bad relation with 6-BA and NAA and the matching ratio of the two directly affected the regeneration frequency of stem segment. Tne effect of 6-BA concentration on the formation of callus was extremely significantly and the effect of NAA was not obvious. Tne rooting time of MS ＋ NAA 0.3 mg/L was shortest,about 15 d, with much roots and better root system and the transplanting survival rate reached 95%. [ Conclusion] Tne optimum medium for the induction of tender stem segment and the propagation of adventitious buds in Dracaena cambodiana was MS ＋ 6-BA 6.0 mg/L ＋ NAA 0.5 mg/L and its propagation coefficient reached over 10.The optimum rooting medium was MS＋ NAA 0.3 mg/L.