雷公藤多甙对健康人单个核细胞IL－6和IL－8产生的影响

Effect of Glucosidorum Tripterygii Tororum on Interleukin-6 and Interleukin-8 on Human Peripheral Mononuclear Cells of Healthy Subjects

目的：探讨雷公藤多甙的抗炎作用机理。方法：应用不同的细胞培养技术，观察雷公藤多甙对健康人周围血单个核细胞产生细胞介素（Ｉｎｔｅｒｌｅｕｋｉｎ，ＩＬ）６及ＩＬ－８的影响，并与氢化可的松进行比较。结果：（１）两种药物均能抑制脂多糖诱导的ＩＬ－６、ＩＬ－８产生；剂量＞１μｇ／ｍｌ时，雷公藤多甙的作用较明显；（２）在单个核细胞培养条件下，雷公藤多甙的作用与氢化可的松相似，但在全血细胞培养时，此作用比氢化可的松弱。结论：（１）雷公藤多甙抑制ＩＬ产生的作用呈剂量依赖性；（２）在不同的细胞培养条件下，ＩＬ对药物的敏感性不同；（３）雷公藤多甙的抗炎作用与其抑制产生ＩＬ有关，其作用机理可能与糖皮质激素不同。

To explore the effect of glucosidorum Tripterygii tororum (GTT). Methods:Using different techniques of cell culture, the function of GTT in comparing with that of hydrocortison (HC) on interleukin (IL)-6 and-8 production by peripheral mononuclear cells of headthy Subjects was investigated. Results: (1) Both of GTT and HC inhibited IL-6, IL-8 production, the effect of GTT being stronger when the dosage was higher than 1 μg/ml;(2) In the isolated cells culture, the effect of GTT was similar to that of HC, but in the whole blood culture, the effect of GTT was much weaker than that of HC. Conclusions:(1) GTT inhibited interleukin production in a dose-dependent manner; (2) The sensitivity of interleukin to drugs was different in different culture conditions; (3)The anti-inflammatory effect of GTT was related to the interleukin inhibition, but the mechanism might be different from that of HC.