中药胎宝抑制抗精子抗体的实验研究

An Experimental Study on Inhibitory Effect of Chinese Medicine Tai-bao on Antisperm Antibody

目的：测定中药胎宝对生殖免疫的作用。方法：将人精子膜抗原注入雌性小鼠腹腔，测定精子抗体为阳性者确定为免疫不育动物模型。分别灌胃中药胎宝高剂量（４６．８ｇ·ｋｇ－１·ｄ－１）、低剂量（３１．２ｇ·ｋｇ－１·ｄ－１），并以强的松（６ｍｇ·ｋｇ－１·ｄ－１）和生理盐水作对照，观察胎宝抑制实验小鼠抗精子抗体、促进妊娠的作用。结果：生理盐水组受孕率３８．８９％，强的松组４７．０６％，胎宝高、低剂量组分别为７０．００％、７５．００％。胎宝低剂量组与生理盐水组比较有显著性差异（Ｐ＜０．０５）。胎宝低剂量组孕鼠平均着床数（１０．９３±２．９１）明显高于强的松组（６．７５±４．５９）。酶联免疫吸附试验（ＥＬＩＳＡ）和微量细胞毒试验测定抗精子抗体，显示胎室组精子抗体下降，胎宝高剂量组和强的松组细胞毒性百分率均显著低于生理盐水组（Ｐ＜０．０５，Ｐ＜０．０１）。结论：中药胎宝对生殖免疫有调节作用，能抑制精子细胞毒抗体，从而促进免疫不育小鼠生育力。

to investigate whether Chinese medicine Tai-bao could inhibit antisperm antibody in experimental mice. Methods: The experimental immunoinfertility mice were due to antisperm antibody induced by injection of human sperm membrane antigens. The experimental immuno-infertile mice used in the present study were divided into four groups including Tai-bao high dose group (46.8g·kg-1·d-1 ), Tai-bao low dose group (31.2g·kg-1·d-1 ), prednisone group and normal saline group. The enzyme linked immune sorbent assay (ELISA) and microcytotoxic assay were used for detection of antisperm antibody. The change of levels of antisperm antibody before and after treatment, pregnant rate, and the number of implantation were investigated in tested mice. Results:The pregnant rates in normal saline group, prednisone group, Tai-bao high dose group and low dose were 38. 89% , 47. 06% , 70. 00% and 75. 00% respectively. The rate of pregnancy in Tai-bao low dose group was significantly higher as compared with normal saline group (P<0. 05). The rate of implantation in Tai-bao low dose group was significantly higher than that in prednisone group (P <0. 05). The results of detection of the cytotoxic antibody to sperm showed that cytotoxic percentages in Tai-bao high dose group (63. 0±10. 3 % )and prednisone group (56. 3±13. 7 % ) were significantly lower (P<0. 05 and P<0.01 ) than that in normal saline group (72. 84±5. 05 % ). Conclusion: Chinese medicine Tai-bao possesses regulatory effect on reproductive immune function, inhibitory effect on antisperm cytotoxic antibody, and promoting effect on pregnancy.