人参皂甙Rb_1对肺缺血再灌注损伤的保护作用

Protective Effects of Ginsenoside Rb 1 on Lung Ischemia Reperfusion Injury

目的探讨人参皂甙Ｒｂ１对肺缺血再灌注损伤的保护作用。方法按肺移植供肺获取和保存的方法，对３５只家兔的肺分别获取，保存；然后采用体外装置，建立体外肺缺血再灌注损伤模型。在即将再灌流前，将不同剂量的Ｒｂ１加入到５０ｍｌ再灌流血液中。结果Ｒｂ１可使肺组织中超氧化物歧化酶（ＳＯＤ）含量升高，丙二醛（ＭＤＡ）含量降低；使肺动脉压（ＰＡＰ）降低，湿肺干肺比重降低和改善肺组织病理变化。Ｒｂ１在再灌流血液中浓度为８０ｍｇ／Ｌ时，已有明显效果。结论Ｒｂ１对肺缺血再灌注损伤具有明显的保护作用。

Objective To determine the protective effects of ginsenoside Rb 1 on lung ischemia reperfusion injury.Methods The lungs of 35 rabbits were harvested respectively and preserved for 24 hours according to the way of lung transplantation. Then the lungs were reperfused using an ex vivo model respectively to establish the models of lung ischemia reperfusion injury. Just before the reperfusion we added the various doses of Rb 1 to the infusate blood. Results Rb 1 can increase lung tissue SOD contents, decrease lung tissue MDA contents, decrease PAP, improve lung edema and histological manifestation. The optimal concentration of Rb 1 is 80mg/L in infusate blood.Conclusion The Rb 1 provided significant protective effects on ischemia reperfusion injury of the lung.