人非小细胞肺癌中p16基因产物表达研究

A Study on the Expression of p16 Gene Product in Human non small cell Lung Cancer

目的探讨人非小细胞肺癌中ｐ１６基因表达产物的表达及其临床意义。方法应用免疫组织化学ＬＳＡＢ法检测７０例人非小细胞肺癌组织ｐ１６基因产物的表达水平，并以２０例正常肺组织标本作对照。结果肺癌组织ｐ１６基因产物阳性表达率为６１．３８％，癌旁肺组织为８９．１４％，正常肺组织为８８．２４％。肺癌中ｐ１６表达水平降低的程度与肺癌细胞分化、原发肿瘤大小和肺癌转移有密切关系（Ｐ＜０．０１或Ｐ＜０．０５），而与肺癌病期、组织学类型、肿瘤部位和患者年龄无明显关系（Ｐ＞０．０５）。结论研究结果表明ｐ１６基因可能参与调控肺癌的发生、发展和转移过程。

Objective To explore the expression of p16 gene product and it's clinical significance in human non small cell lung cancer.Methods Expression level of p16 gene product was detected in 70 non small cell lung cancer samples and 20 normal lung tissues as control by immunohistochemistry.Results The expression level in lung cancer (61.38%) was significantly lower than those in adjacent nontumor lung tissue(89.14%) and normal lung tissue (88.24) ( P <0.01). The degree of decreased expression of p16 gene product in lung cancer was closely related to cell differentiation of the tumor, size of the primary tumor, and metastasis of the cancer ( P <0.01 or P <0.05), but not to stages of the tumor, histological classification of the tumor, location of the cancer, and ages of the patients ( P >0.05).Conclusion In this study, the results suggest that p16 gene may be involved in the oncogenesis, development and metastasis of human non small cell lung cancer.