伴血吸虫病的结肠癌和息肉中c-erbB-2表达及AgNOR的意义

Expression of c erbB 2 oncogene and AgNOR in colon cancer and polyp with schistosomiasis

目的研究并存血吸虫病的结肠癌和肠息肉中ｃｅｒｂＢ２的表达，ＡｇＮＯＲ颗粒的数量及特点，探讨血吸虫病与结肠癌发生的关系．方法应用免疫组织化学ＬＳＡＢ法对３３例并存血吸虫病的结肠癌和肠息肉（结肠癌２１例，伴有肠系膜淋巴结转移１７例；肠息肉１２例）和２０例无血吸虫病的结肠癌和肠息肉（结肠癌１０例，伴有肠系膜淋巴结转移４例；肠息肉１０例，进行ｃｅｒｂＢ２标记，并作ＡｇＮＯＲ染色．结果肿瘤细胞中癌基因ｃｅｒｂＢ２的表达呈明显异质性．２１例并存血吸虫病的结肠癌中７例阳性（３３３％），其中５例伴有肠系膜淋巴结转移者均为强阳性；１０例无血吸虫病的肠癌中３例阳性（３００％），其中２例伴有肠系膜淋巴结转移者均为强阳性；１２例并存血吸虫病的肠息肉中５例阳性（４１７％）；１０例无血吸虫病的肠息肉中仅１例阳性（１００％）．并存血吸虫病的肠息肉中ｃｅｒｂＢ２的表达明显高于无血吸虫病的肠息肉（Ｐ＜００５）ＡｇＮＯＲ／核均数示２１例并存血吸虫病的肠癌为５８６±１７７；１０例无血吸虫病的肠癌为４７５±１１３；１２例并存血吸虫病的肠息内为３５２±１２２，其中５例ｃｅｒｂＢ２阳性的肠息肉为３９４±?

AIM To study the expression of c erbB 2 and AgNOR in colon cancer and polyp with schistosomiasis, and the relationship between schistosomiasis and colon cancer. METHODS From 1992 to 1995, 33 cases of colon cancer and polyp with schistosomiasis and 20 cases of colon cancer and polyp without schistosomiasis were tested for c erbB 2 and AgNOR. RESULTS c erbB 2 positive rate in colon cancer with schistosomiasis was 33 3% (7/21), and in colon cancer without schistosomiasis was 30% (3/10). c erbB 2 positive rate in colon polyp with schistosomiasis was 41 7% (5/12), and in colon polyp without schistosomiasis was 10 0% (1/10). The difference between the two groups was significant ( P <0 05). AgNOR number per nucleus in colon polyp with schistosomiasis was 3 52±1 22, and in colon polyp without schistosomiasis was 2 47±0 89, the difference being significant ( P <0 05). CONCLUSION Colon polyps accompanied by schistosomiasis infection are charaterized by precancerous lesions. Malignant latency in c erbB 2 positive colon cancer was high.