海参中总皂苷含量测定方法的研究

Study on the determination of total triterpene glycosides in sea cucumbers

目的建立一种海参中总皂苷含量的测定方法。方法以海参三萜皂苷Echinoside A作为标准品,采用香草醛-高氯酸作为显色体系,以60%乙醇提取,正丁醇萃取得海参样品中总皂苷,利用标准曲线测定了11种市售海参样品的总皂苷含量,并采用皂蛋比(皂苷/蛋白质)作为指标讨论了海参品种、生长环境、加工方法等因素对皂苷含量的影响。结果确定测定波长为560nm,标准曲线为y=1.3414x-0.0077,该方法在0～0.5mg范围内呈现良好的线性关系(R2=0.9994)。本测定方法的加样回收率为(99.01±2.82)%,RSD为4.68%。结论方法简便,重现性好,可作为海参中总皂苷含量测定的方法。

Objective A method was developed for determination of the total triterpene glycosides in different sea cucumbers. Methods A holostane triterpene glycoside called Echinoside A was taken as the reference standard. Triterpene glycosides were reacted with vanillin and perchloric acid. The sea cucumbers were extracted by 60% ethanol and partitioned between water and n-butanol to gain the total triterpene glycosides. The contents of total triterpene glycosides in 11 kinds of sea cucumbers were determined with the standard curve. And the relationships of the triterpene glycosides content among different sea cucumber species, growth environment, process technique, etc. were discussed by the value of TG/P（triterpene glycosides content / protein content）. Results The determination wavelength was confirmed to be 560nm and the standard curve was determined as y=1. 3414x-0. 0077. The absorbance was of a good linearity in the mass range of 0-0.5mg with R2 =0. 9994. The recovery of this method was （99.01;2.82）% and the relative standard deviation was 4. 68%. Conclusion The method has been proved to be convenient, reliable and suitable for the analysis of triterpene glycosides in sea cucumbers.