摘要
目的建立测定鱼露中章鱼胺含量的高效液相色谱学方法。方法采用phenomenex luna C18柱,以0.02mol.L-1柠檬酸-0.02mol.L-1磷酸二氢钠(7:3)为流动相,检测波长λ=274nm。结果章鱼胺在0.0057～50μg.mL-1范围内,浓度与峰面积线性关系良好(r=0.9991),平均加样回收率为104.4%,RSD为1.53%。最低检出浓度为5.7ng.mL-1。结论本法操作简便,结果可靠,可用于鱼露中章鱼胺及相关物质含量的测定,鳀鱼鱼露含章鱼胺1055μg.mL-1。
Objective To establish a HPLC method for the determination of octopamine in fish sauce. Methods A phenomenes luna C18 column was used. The mobile phase was 0. 02μg · mL^-1 citric acid-0. 02μg · mL^-1 sodium dihydrogen phosphate （7 ： 3, v/v） and detection waveleng, h was 274 nm. Results The linear range of octopamine was 104. 0%, RSD= 1.53%. The detection limit was 5.7ng · mL^-1. Conclusion This method is simple, rapid and reliable. It could be used for the determination of octopamine and its related substances in fish sauce. The content of octopamine in Engraulisjaponicus sauce is 1055μg · mL^-1.
出处
《中国海洋药物》
CAS
CSCD
2008年第1期43-45,共3页
Chinese Journal of Marine Drugs
基金
江苏省高技术研究计划项目(BG2006013)
