大熊猫幼兽腹泻粪便分离出的轮状病毒鉴定

Isolation and identification of rotavirus from giant panda cubs

11只5～11月龄的断奶大熊猫幼兽先后患一种急性传染性、顽固性腹泻病,1只大熊猫幼兽在发病4d后死亡,相同饲养条件下由母兽哺育的同龄大熊猫幼兽未见发病。取发病大熊猫幼兽粪便、血液以及呕吐物进行细菌学和寄生虫学检查,未检出病原;用轮状病毒粪便抗原ELISA快速诊断,受检的8只大熊猫幼兽腹泻便都多次检出阳性结果。采集发病大熊猫的病料进行病毒病原分离,从腹泻大熊猫幼兽的粪便中分离出病毒颗粒,通过电镜形态、理化特性和中和试验鉴定,确认分离病毒为轮状病毒。

11 giant panda cubs （ age ranged from 5 to 11 months） suffered an acute, obstinate and infectious diarrhea, 1 panda cub aged 6 months died just 4 days after the onset of clinical signs. All of the sick cubs were these animals weaned from their mothers. Their peers who were still being nursed by their mothers seemed unaffected even in the same environment. Samples from all of these sick panda cubs had been cultured for bacteria and examined for parasites, but results of these examinations were negative. In a survey performed for viral pathogens, feces from 8 of the 11 panda cubs were tested for rotavirus by an ELISA kit, most of the samples from each of the 8 cubs were positive. Fecal samples were collected for isolation of virus. Rotavirus was isolated from these samples and identified by electronic microscopy, physical and chemical characteristics, and serum neutralization test.