东北虎源等孢球虫的发现

Discovery of Isospora from Panthera tigris altaica

对东北虎粪便进行虫卵检查,分离获得了自然感染的球虫卵囊。对球虫卵囊的形态和经培养发育后的各阶段的卵囊形态进行了观察。东北虎源球虫卵囊符合等孢属球虫的形态学特征。根据Genbank上发表的猫等孢球虫18S rDNA序列(L74671)设计一对特异性引物,利用PCR技术对东北虎源球虫的18S rDNA进行了扩增。扩增得到一段360bp的DNA片段,并对该片段进行了序列测定。测序结果和其它17种原虫的相应序列进行序列同源性比较分析,分析显示东北虎源球虫和其它等孢属球虫单独聚为一类。结合对东北虎源球虫的卵囊形态、卵囊发育情况及序列分析结果,确定其为等孢属球虫。

Coccidia oocysts were obtained from Panthera tigris altaica infected naturaly. The morphology of the oocysts were observed as well as the morphology of varied stages by incubated it in the laboratory. Based on the sequence of Isospora fells （L74671} published on the Genbank, a pair of specific primers were designed. By PCR amplification, a 360 bp DNA from 18S rDNA of coccidia from Panthera tigris altaica was produced, and the purified product was directly sequenced. Based on the results of morphology, sporalation and phylogenetic analysis, it was concluded that the coccidia from Panthera tigris altaica was Isospora.