N-乙酰半胱氨酸和维生素C抗大鼠急性肺损伤作用的比较研究

Comparation Study on the Effect of N-acetyl-cysteine and Vitamin C Against Acute Lung Injury in Rat

目的比较研究N-乙酰半胱氨酸(NAC)和维生素C(Vc)抗大鼠急性肺损伤(ALI)作用。方法采用大鼠油酸(OA)所致急性肺损伤模型,随机分为正常对照组(NS组)、ALI模型组(OA组)、维生素C干预组(Vc组)、NAC干预组(NAC组)。观察NAC和Vc对大鼠急性肺损伤时MDA,SOD和GSH-PX水平,病理改变,肺系数,免疫组化iNOS等的影响。结果NAC组和Vc组可明显降低损伤所致肺系数、iNOS表达、组织MDA等水平的升高;同时,又能显著升高肺组织SOD、GSH-PX水平,且Vc组和NAC组之间无显著性差别(P>0.05)。结论抗氧化剂NAC和Vc可通过抑制MDA、iNOS表达,改善SOD、GSH-PX等方式减少氧自由基的产生,减轻肺损伤程度,对油酸所致急性肺损伤后的肺纤维化有一定防护作用。

OBJECTIVE To compare the effect of N-acetyl-cysteine and Vitamin C against acute lung injury in rat. METHODS Using acute lung injury rat induced by oleic acid, animals were randomly divided into four groups and there were ten rats in each group： control group （NS group）, ALI model group （OA group）, NAC pretreatment group （NAC group） and Vc pretreatment group （Vc group）. Malondialdehyde （MDA）, superoxide dismutase （SOD）, glutathione peroxidase （GSH-PX） and lung coefficient were detected. Simultaneously, the pathological slices and the immunohistochemical staining of the iNOS expression in lung tissues were observed. RESULTS Comparing with the control group, the lung coefficient, MDA and expression of iNOS were obviously downregulated by N-acetyl-cysteine and Vitamin C. N-acetyl-cysteine and Vitamin C significantly increased the levels of SOD and GSH-PX. CONCLUSION Our present results suggest that N-acetyl-cysteine and Vitamin C might inhibit the level of MDA and the expression of iNOS, increase the levels of SOD and GSH-PX, and hence reduce releasing of free radicals. N-acetyl-cysteine and Vitamin C had a protective effect on acute lung injury.