正交试验法优选三叶青中总黄酮的提取工艺

Orthogonal Test for Optimizing the Extracting Method of Total Flavonoids from Radix Tetrastigmae

目的优选三叶青中总黄酮的提取工艺。方法以三叶青总黄酮为指标,采用单因素考察和正交试验相结合的方法,对乙醇浓度、乙醇用量、提取时间、提取次数4个影响因素进行研究,优选提取工艺。结果乙醇浓度对三叶青总黄酮的提取影响较大,其他3个因素影响程度排序为:提取次数(提取时间(乙醇用量。结论最佳提取工艺参数为:50%乙醇、15倍量、回流提取3次、以及每次2 h。

OBJECTIVE To optimize extraction technology of the total flavonoids from Radix Tetrastigmae. METHODS In the method of one-way and orthogonal test, the ethanol concentration and amount, extraction time and number of extractions were studied with the total flavonoids from Radix Tetrastigmae as the target of the test. RESULTS The ethanol concentration had great impact on the target, The order of the other 3 factors that affected the flavonoid extractions was number of extractions 〉 extraction time 〉 amount of ethanol, CONCLUSION The parameters of the best extraction technology are as follows ： 50% ethanol, 15 times the weight of the dried material, refluxing and extracting 2 h for 3 times.