反胶束溶液化学发光法测定甲磺酸培氟沙星

Chemiluminescence analysis of pefloxacin mesylate with reversed micellar

在酸性条件下，甲磺酸培氟沙星分子中氮原子被质子化后与阴离子AuCl4^-形成离子缔合物，该缔合物被二氯甲烷带入鲁米诺的氯化十六烷基三甲基铵（CTAC）反束溶液中，离解出来的AuCl了立即与鲁米诺产生化学发光．在一定浓度范围内，发光强度与甲磺酸培氟沙星的含量成线性关系，从而间接测定甲磺酸培氟沙星的含量．在优化的试验条件下，线性范围为0．001～10×10^-6g·mL^-1，检出限（3σ）为0．02×10^-9g·mL^-1，对浓度为1．0×10^-6g·mL^-1的甲磺酸培氟沙星进行11次平行测定，RSD为2．4％．该法已成功用于片剂、注射液和生物体液中甲磺酸培氟沙星的测定．

In acidic condition, the nitrogen in pefloxacin mesylate was easily protonated and formed ion-complexes with negative ion AuCl4^-, the ion-complexes were extacted and carried by dichloromethane, when the ion-complexes entered a reversed micellar of cetyltrimethylammonium chlorine containing luminol, the dissociated AuCl4^- reacted with luminol and produced an analytical chemiluminescnece signal. In the optimum conditions,the linear range is 0. 001 to 10 × 10^-6 g·mL^-1 and the limit of detection is 0.02 × 10^-6 g·mL^-1 with a relative standard deviation （ n = 11） of ca. 2.4% for 1.0 × 10^-6 g·mL^- 1 pefloxacin mesylate. The method has been applied to the determination of the studied drug in tablets, injection and biological fliuds successfully.