p16与C-myc在喉鳞状细胞癌中的表达意义

Significance of p16 and C-myc expression in the laryngeal squamous cell carcinoma

目的：研究p16和C－myc在喉鳞状细胞癌中的表达意义。方法：应从p16和C－myc单克隆抗体，通过免疫组织化学法检测6例正常喉粘膜和47例喉鳞癌中p16和C－myc的表达。结果：6例正常喉粘膜组织中未发现p16和C－myc蛋白阳性表达，47例喉鳞癌中p16和C－myc阳性表达率分别为48．9％（23／47）和57.4％（27／47），与性别、年龄和肿瘤发生的部位无相关性（P＞0．05），与临床分期和病理分级相关（P＜0．05）；C－myc还与颈淋巴结转移相关（P＜0．05）。结论：p16和C-myc蛋白阳性表达在喉鳞癌的发生和发展中起重要作用，可作为评价喉鳞癌预后的新指标。

ObJective: To study the clin1cal slgnificance of p16 and C-myc expression in the laryngeal squamous cell carclnoma (LSCC ). Method: Immunohisto-chemical detection of p16 prote1ns and C-myc protelns,in 47 cases of LSCC and 6 cases of normal tissues from the larynx, were conducted in paraffin-embeded sections. Results: No staining was found in 6 cases of normal tissue;The positive expression of p16 and C-myc proteins expression rates did not correlate with sex,age and position of the tumors(P>0.05 );but related to the staging and grade of LSCC(P<0. 05). The expression of C-myc proteins were higher in the case with lymph nodal metastasis than that without lymph nodal metastasis (P < 0. 05 ). Conclusion: The expression of p16 and C-myc protein may play an important role in the development of LSCC,and may be a new indicator to assess the prognosis of LSCC.