小鸡单离听毛细胞的分离及其形态学观察

Dissociation and morphological observation of hair cells from chick basila papilla

用胶原酶孵育后机械分离15只小鸡的听毛细胞，平均每侧可分离428个。对其中5只鸡的215个活性良好的单离毛细胞进行了形态学测量，其中高毛细胞164个，长20．22±3．32μm，宽9．93±2．01μm，矮毛细胞30个，长10．97±2．29μm，宽14．8±2．29μm，中间型毛细胞21个，长宽相等，均为11.93±1.74μm。用双醋酸荧光素-碘化丙啶对另外5只鸡单离毛细胞进行染色，在荧光显微镜下观察细胞的变性过程。鸡断头3h内，活性良好的细胞着非常明亮的绿色荧光，到5h，活性细胞呈现不清晰的绿影。实验结果表明，判断单离毛细胞的活性，应将形态学观察标准与细胞染色结合起来。

Hair cells were dissociated from basiIa papilla of 7 to 10 week post-hatched chicks (n = 15 ) by collagenase digestion followed by trituration. Morphological measurements of 215 viable hair cells from 5 chicks were performed. The average dimensions of the cells were:length 20.22±3. 32μm,width 9. 93± 2.01μm of tall hair cells (n = 164);length 10. 97± 2. 29μm,width 14.8 ±2.29μm of short hair cells (n = 30); length = width = 11. 93 ±1. 74μm of intermediate hair cells (n = 21 ). Using fluorescein diacetate and propidium iodide, viability of isolated hair cells from another 5 chicks was determined. With excitatory light, fluorescence of viable cells were bright green in 3 hours,while the f1uorescence of live cells were attenuated to such an extent that light green shadows were showed up to 5 hours after chicks were decapitated. The results suggested that the criterion of morphological observation and dying of cells should be combined to determine viability of isolated hair cells.