超高压提取丹参素的研究

Ultrahigh pressure extraction of Danshensu

该文研究常温下超高压提取丹参素的最优工艺。采用超高压常温提取,正交试验对丹参素超高压提取工艺优化,高效液相色谱法测定丹参素的含量,并与传统的热回流提取法进行比较。结果显示优选的最优工艺为:提取压力300 MPa,溶剂乙醇浓度80%,溶液pH 13,浸泡时间16 h,在此条件下丹参素得率为0.465%。超高压提取不仅得率高、时间短、耗能低,而且提取产物易于分离、纯化,同时超高压提取可以维持在低温下进行,特别适宜于受热不稳定化合物的提取,该技术适合中药有效成分的快速提取。

The optimum extraction technology of Danshensu under normal temperature by ultrahigh pressure technique was investigated. The Danshensu was extracted with ultrahigh pressure under normal temperature. The effects of various extraction conditions on extraction yield were optimized by orthogonal test design. The ultrahigh pressure extraction was compared with the conventional extraction method of refluxing boiling solvents under atmospheric pressure ,and the yields of Danshensu were determined by HPLC. The results show that the optimum parameters are as follows： pressure 300 MPa, 80% ethanol as the solvent, pH 13 and 16 h of dipping time .Under the optimum conditions, the extraction yield of the Danshensu is 0.465%. Ultrahigh pressure extraction is not only more extraction efficient, shorter extraction time, less energy consumption, but also convenient for the recovery and purification of the active ingredients of plant materials. In addition, the ultrahigh pressure extraction can be carried out at lower temperatures and is favorable for the thermally unstable compounds. It is suitable for fast extraction of active ingredients from the Chinese medicine.