京尼平对壳聚糖及明胶的交联反应

Crosslinking Reaction of Chitosan and Gelatin with Genipin

壳聚糖和明胶均具有良好的生物相容性,但作为医用植入材料其力学性能往往不能达到要求.交联是改善其力学性能的常用方法,但传统化学合成交联剂均具有相对较高的细胞毒性.选用天然化合物京尼平为生物交联剂,借助紫外光谱分析方法,对京尼平与壳聚糖的交联反应过程进行跟踪研究,建立了该交联反应机理及动力学的初步模型.对壳聚糖-京尼平和壳聚糖-明胶-京尼平复合体系的力学性能和溶胀性能进行了探讨,并研究了在溶菌酶的存在下壳聚糖-京尼平复合体系的降解行为.结果表明,壳聚糖-明胶-京尼平复合体系的机械性能与京尼平的加入量有明显的关系.

Chitosan and gelatin have good biocompatibility. However, sometimes their mechanical performances cannot match the requirement for medical implanted materials. Crosslinking is a usual effective method to enhance the mechanical strength of polymers. The conventional crosslinkers of chitosan or gelatin are aldehydes, but their physiological toxicities limit the application of chitosan and gelatin. Here genipin, a biocrosslinker, was selected to take the place of aldehyde. The crosslinking mechanism and kinetics of chitosan and genipin were investigated by UV spectra, and the corresponding models were established especially. The mechanical properties, swelling degree and degradation behaviors of the chitosan-genipin and chitosan-gelatin- genipin gels were also researched. Results show that the mechanical performance of chitosan-gelatin complex system crosslinked by genipin could be improved in a certain range by changing the crosslinking degree.