重组白细胞介素2对骨髓造血祖细胞体外增殖的影响

Effect of recombinant interleukin 2 on vitro proliferation of hematopoietic progenitor cells from human bone marrow

为了了解重组白细胞介素２对骨髓造血祖细胞体外增殖的影响，应用体外半固体培养法研究了ｒｈＩＬ－２及其与ＧＭ－ＣＳＦ和Ｅｐｏ组合对骨髓祖细胞集落形成的调节作用。结果：ｒｈＩＬ－２单独对骨髓造血祖细胞的体外增殖无刺激作用；当其与ＧＭ－ＣＳＦ联合应用时，在浓度为１０．０×１０４～４０．０×１０４Ｕ·Ｌ－１时，ＣＦＵ－ＧＭ集落数明显高于ＧＭ－ＣＳＦ对照组；当其与Ｅｐｏ联用时，在浓度为１０．０×１０４～４０．０×１０４Ｕ·Ｌ－１时ＣＦＵ－Ｅ和ＢＦＵ－Ｅ集落数明显高于Ｅｐｏ单用组；但当其浓度升至１０．０×１０５Ｕ·Ｌ－１时，各种集落数均明显减少。重组人白介素２（ｒｈＩＬ－２）对骨髓造血祖细胞体外增殖的研究结果显示，在一定的浓度范围内，ｒｈＩＬ－２与ＧＭ－ＣＳＦ和Ｅｐｏ联合对骨髓造血祖细胞的体外增殖具有协同的刺激作用，单独应用无集落形成能力，大剂量应用可能会抑制骨髓造血。

In order to determine the regulation effects of rhIL 2 on hematopoietic progenitor cells from bone marrow. The effects of rhIL 2 on proliferation of colonies from CFU GM, CFU E and BFU E in vitro on were studied in a semisolid culture. The results showed that rhIL 2 couldnt stimulate the formation of colonies from CFU GM, CFU E and BFU E alone, but it might synergistically promote hematopoiesis within a proper concentration(10 0×10 4～40 0×10 4 U·L -1 ) with GM CSF and Epo. High dose of rhIL 2 exerted an inhibitory effect on hematopoiesis. Our data suggested that ABMT using ABM activated by rhIL 2 combining with other hematopoietic growth factors may decrease the rate of relapse in leukemia or other solid tumors, and also promote hematopoietic and immunologic reconstitution.