摘要
目的:探讨桂枝挥发油对LPS致大鼠急性肺损伤模型蛋白酪氨酸激酶活性的影响;方法:采用LPS 1 mg/kg尾静脉注射构建大鼠急性肺损伤模型,取肺组织,采用ELISA方法检测不同干预组肺组织中PTK含量,比较组间差异;结果:研究表明,SD大鼠正常肺组织中PTK含量为(44.21±31.51)pmol/mL;模型组含量为(173.58±97.95)pmol/mL,较空白组显著增高,具有极显著的统计学意义(P<0.001);VORC高、中、低3个剂量组PTK含量均较模型组显著降低(P<0.05),有一定的量效关系。结论:桂枝挥发油能够抑制LPS所致大鼠急性肺损伤肺组织中PTK的异常增高,对PTK活性的抑制可能是桂枝挥发油发挥抗炎作用的主要机制之一。
Objective： To study the VORC （the Volatile oil of Ramulus Cinnamomi ） on activation of PTK in the pulmonary tissue of acute pneumonia rat model. Method： The rat model of acute pneumonia was made. After treatment by VORC, The content of PTK in the lung tissues of the rat was determined with ELISA technic. Result： Results show that the content of PTK is （44. 21 ±31.51） pmol/mL in the pulmonary tissue of control group, that of （ 173.58±97.95） pmol/mL in model group, which is higher than that of control group （ P 〈 0. 001 ） ; The contents of PTK of all three VORC groups are lower significantly than that of model group, which, six hour after LPS injected through cauda mainline are significantly higher than that of the normal group （P 〈 0. 05 ）. Conclusion： VORC can inhibit the activation of PTK in the model of rat acute pneumonia. Results showing the anti - inflammation effect of VORC has some relation with inhibiting of PTK activation.
出处
《成都中医药大学学报》
2007年第4期28-30,共3页
Journal of Chengdu University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(NO.30472201)
关键词
桂枝挥发油
急性肺损伤
蛋白酪氨酸激酶
实验研究
The Volatile oil of Ramulus Cinnamomi
Acute pheumonia
PTK activation
Lahoratory research
