p53蛋白在人肝细胞癌及其肝内转移灶和癌栓中的表达及意义

The Expression of P53 Protein in Human Hepatocellulor Carcinoma,lts lntrahepatic Metastatic sites and lts Venous tumor Embolus

应用ＬＳＡＢ法检测了５２例人肝细胞癌（ＨＣＣ）及其肝内转移灶和癌栓中Ｐ５３的表达水平，并对Ｐ５３表达与ＨＣＣ浸润转移和预后关系进行了前瞻性研究。结果表明，在原发癌灶、肝内转移灶和癌栓中Ｐ５３表达无显著性差异；在分化较差和包膜浸润的ＨＣＣＰ５３高表达；转移组原发癌癌栓较单发癌癌栓Ｐ５３高表达；Ｐ５３高表达的ＨＣＣ术后复发率较高，预后较差。提示Ｐ５３基因及其蛋白产物在ＨＣＣ浸润播散过程中起重要调控作用，Ｐ５３可用作ＨＣＣ预后评估的判定指标。

By LSAB immunohistochemical method,the relations of expression of p53 Protein,intrahepatic invasiveness and metastasis and prognosis of human hepatocellular carcinoma (HCC)were studied in 52 cases,among which there were 30 cases with intrahepatic metastatic sites and 34 cases with venous tumor embolus.The levels of p53 oxpression were significantly higherin primary sites,intrahepatic metastatic sites and venous tumor embolus than those of adjacent nontumor liver tissure (P<0.001),The levels of p53 expression were higher in poor Edmondsons histopathological classfication and in the carcinotissues of invasive capsule (P<0.05).In the venous tumorembolus of single Primary site the levels of P53 expression were higher than those with the intrahepatic metastatic Primary sites (P<0.05),p53 overexpression was associated with the higher recurrence rate after surgical resection.It is suggested that p53 gene and p53 Protein might play an important role in infiltrative dissemination of HCC.P53 Protein might be regarded as an index of evaluating prognosis of the HCC.