O型人血清中交叉反应性抗体的初步研究

The preliminary study of cross reacting antibody in group O sera

对Ｏ型人血清中ＩｇＭ和ＩｇＧ交叉反应性抗体的性质作了初步研究。实验证明：Ｏ型人血清经Ａ型细胞和Ｂ型细胞吸收后，血清中除抗Ａ和抗Ｂ被完全吸收外，抗Ｂ和抗Ａ效价也下降１～２个稀释度。Ｏ型人血清中除有抗Ａ和抗Ｂ外，还有一种不可分离的抗Ａ，Ｂ。交叉反应性ＩｇＭ抗Ａ，Ｂ的凝集强度一般效价在２～８。Ｏ型血清经２－ｍｅ处理后，ＩｇＧ抗Ａ，抗Ｂ效价在６４～２５６之间，经Ａ型细胞和Ｂ型细胞吸收后，吸收液中抗Ｂ和抗Ａ效价也分别下降。证明交叉反应性抗Ａ，ＢＩｇＭ及ＩｇＧ二种免疫球蛋白都有，但ＩｇＧ抗Ａ，Ｂ的放散能力比ＩｇＭ抗Ａ，Ｂ明显减低，其机制尚待进一步研究。还证明交叉反应性抗Ａ，Ｂ都能被血型物质中和。

In this paper,the character of IgM and IgG cross reacting antibody in group O sera was investigated. The tests indicated that the absorption of O sera with A or B cells could not only absorb completly the agglutinin of group O sera against A or B cells,but also reduce the titre of group O sera against B or A cells. It showed that serum from group O individual contains not only anti A and anti B,but also anti A,B.Anti A,B antibody activity cannot be seperated into a single specificity. The titre of IgM cross reacting anti A,B was usually between 2 8. When the O sera were treated with 2 me,the titre of IgG anti A,B were between 64 256.When the O serum was absorbed with A and B cells,the titre of anti B and anti A reduced,too. It was demonstrated that the IgM and IgG cross reacting anti A,B were presented, but the titre of eluate of IgG anti A,B was obviously lower than IgM anti A,B. It was aslo demonstrated that cross reacting anti A,B can be neutralized by blood group substance.