TRFIA与ELISA法检测乙肝病毒血清学标志物结果的比较

Comparison of HBV Serum Marker Results between TRFIA and ELISA

目的探讨时间分辨荧光免疫测定法(TRFIA)定量检测乙型肝炎血清学标志物(HBVM)与酶联免疫吸附试验(ELISA)定性检测HBVM的关系及其临床意义。方法采用TRFIA和ELISA法分别检测161份血清标本HBVM。结果对于高浓度的HBVM血清,两种方法检出率有较高的一致性,但是对于稍高于临界值的血清,TRFIA检出率明显高于ELISA。结论TRFIA检测HBVM比ELISA法敏感。

Objective To explore the clinical value and relationship between time-resolved fluoroimmunoassay（TRFIA） to detect HBV M in quantity and enzyme linked immunosorbent assay（ELISA） to detect HBV M in quality. Methods 161 serum specimen were collected to detect HBV M by TRFIA and ELISA. Results The results of TRFIA were accorded to that of ELISA for high titre HBV M specimen. The positive results of TRFIA were higher than that of ELISA for nearly cut-off value HBV M specimen. Conclusion TRFIA is more sensitive than ELISA.