核转录因子EBF3 mRNA在肝癌和食管癌组织中表达水平的研究

Study on Expression of Early B Cell Factor 3 mRNA in Tissues of Primary Hepatocellular and Esophageal Carcinoma

目的研究核转录因子EBF3 mRNA在肝癌和食管癌组织中表达水平及其在肿瘤发生中的意义。方法肝及食管组织分别以β2微球蛋白(β2M)和18S rRNA为内参,实时荧光定量RT-PCR方法检测肝癌及食管癌及其远端组织中EBF3 mRNA的含量。结果18例肝癌和配对远端肝组织中EBF3 mRNA与β2M mRNA的对数比值分别为0.52±0.17和0.28±0.23,差异有统计学意义(t=3.56,P=0.0011)。12例食管癌和配对远端食管组织中EBF3 mRNA与18S rRNA的对数比值分别为0.58±0.054和0.22±0.24,差异有统计学意义(t=5.07,P=0.0000)。结论EBF3 mRNA在肝癌和食管癌组织中表达显著增高。核转录因子EBF3可能与肝和食管肿瘤的发生有关。

Objective To investigate the significance of early B-cell factor 3（EBF3） mRNA level in tissues of primary hepatocellular and esophageal carcinoma. Methods Amplification of 18S rRNA and β2 microgluobulin （β2M） were used as controls for RNA integrity for all tissue samples of liver and esophagus . The expression levels of EBF3 mRNA in liver and esophageal cancerous and their paired noncancerous tissues were determined by fluorescence quantitative real-time polymerase chain reaction（FQ-RT-PCR）. Results The ratios of EBF3 mRNA to β2M mRNA in 18 liver cancerous tissues （0. 52±0. 17） were significantly higher than those in distant liver noncancerous tissues（0. 28±0. 23, t=3.56,P=0. 001 1）. The ratios of EBF3 mRNA to 18S rRNA in 12 esophageal cancerous tissues （0. 58±0. 054） were significantly higher than those in distant esophageal noncancerous tissues（0. 22±0. 24,t=5.07,P=0. 000 0）. Conclusion The results suggested that EBF3 mRNA may contribute to occurrence of primary hepatocellular and esophageal carcinoma.