外周血单个核细胞IRF-7 mRNA水平与乙肝病毒慢性感染关系的研究

Study on Relationship between IRF-7 mRNA Expression in PBMC and HBV Chronic Infection

目的探讨外周血单个核细胞(PBMC)IRF-7 mRNA水平与乙肝病毒(HBV)慢性感染的关系。方法应用实时荧光相对定量RT-PCR法分别检测45例慢性乙型病毒性肝炎(CHB)患者(轻度组、中度组以及重度组各15例)和30例正常对照的PBMCIRF-7 mRNA水平。结果CHB患者PBMCIRF-7 mRNA表达水平较正常对照组明显下降(P<0.01);IRF-7 mRNA水平由高到低依次为CHB轻度组、中度组、重度组(P<0.01)。结论CHB患者PBMCIRF-7 mRNA水平明显下降,且与疾病严重程度呈正相关,提示可能与乙型肝炎病毒慢性感染有关。

Objective To study the relationship between IRF-7 mRNA levels in peripheral blood mononuclear cell （PBMC） and HBV chronic infection. Methods Real-time fluorescence relative quantitative RT-PCR was used to quantify IRF-7 mRNA levels in PBMC from 45 chronic hepatitis B（CHB） cases （15 cases of mild, 15 moderate and 15 severe） and 30 normal controls. Results The PBMC IRF-7 mRNA levels in CHB cases were significantly lower than those in control （P〈0. 01）. The levels of IRF-7 mRNA decreased significantly in an order from mild CHB group ,moderate group to severe group （P〈0. 01）. Conclusion The expression levels of IRF-7 mRNA in PBMC of patients with CHB were decreased,and were positively correlated with the degree of CHB,it may be correlated with the HBV chronic infection.