用PCR-SSCP技术检测并鉴定胃粘膜和唾液中的幽门螺杆菌

DETECTION AND DIFFERENTIATION OF HELICOBACTER PYLORI FROM GASTRIC BIOPSY AND SALIVA BY PCR SSCP

运用聚合酶链反应（ＰＣＲ）分别检测来自４８例患者的胃粘膜和唾液标本中的幽门螺杆菌（Ｈｐ）的种系特异性抗原基因，并进一步运用单链构象多态性分析技术（ＳＳＣＰ）鉴定存在于人群之中和同一个体不同部位的Ｈｐ菌株。结果表明：在胃粘膜Ｈｐ阳性的３６例患者中，唾液标本的检出率为７２．２％（２６／３６）。在胃粘膜Ｈｐ阴性的１２例患者中，有２例患者的唾液Ｈｐ呈阳性（１６．７％）。进一步的ＳＳＣＰ分型证实：人群中感染的Ｈｐ菌株可根据其种系特异性抗原基因区分为６种基本带型。而从２６对同时呈阳性的胃粘膜和唾液ＰＣＲ产物经ＳＳＣＰ分型，其中２５例患者呈现相同或相似带型，有１例呈明显差异。上述结果表明同一个体的不同部位存在同种菌株的可能性大，但并不排除混合性感染的可能。

In this study we detected the species specific antigen gene of Helicobacter pylori (Hp) from gastric biopsy specimens and saliva of 48 patients by polymerase chain reaction (PCR), then adopted single strand conformation polymorphism(SSCP) to differentiate Hp strains from population and different sites of the same individual. Among patients of positive gastric biopsy results, the detection rate of saliva was 72.2%(26/36). In 12 patients of negative gastric biopsy results, 2 saliva specimens were positive. Further SSCP analysis demonstrated that in population the infected Hp strains showed 6 different band patterns. SSCP analysis of PCR products from the gastric biopsy specimen and saliva of the same individual showed no significant difference. The results showed that Hp at different sites of one individual probably belongs to the same strain.